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Climate change has been playing a crucial role in altering the precipitation patterns in the southern USA. States like Mississippi, Louisiana, and Alabama have seen increased numbers of extreme events like hurricanes, storms, and heavy rainfall. Therefore, rainfall-induced landslides have been very common in recent years. In Mississippi, due to the prevalence of highly expansive clay soil, slope failure has brought about a huge financial burden for the authority. In order to create resiliency in highway embankments, regular monitoring and early detection of landslide risks are important. The objective of the current study is to evaluate the landslide behavior of highway slopes under changed climatic conditions. One highway slope near Grenada, Mississippi, was selected for the study. The slope has a history of shallow landslide. Remote sensing technology like Light Detection and Ranging (LiDAR) has been utilized to compare the topographical surfaces in different seasons. Electrical Resistivity Imaging (ERI) was performed, and seasonal variations in subsurface moisture contents were obtained from the ERI profiles. In addition, rainwater data of the site location from available open sources were collected. Perched water zones have been detected through the ERI images when there were events of extreme rainfall. A drone mounted with an advanced LiDAR scanning system has been utilized to detect any trend of slope movement in the study site. The LiDAR scan gathered dense point cloud data to construct 3D surfaces and produce topographic maps of the slope. The integration of ERI and LiDAR provides a comprehensive understanding of the climate resilience of highway slopes in the face of climate change. 

The hydro-mechanical behavior of unsaturated soil, particularly expansive soil, is influenced significantly by cyclic wetting and drying. Understanding the soil parameters is crucial when evaluating the performance of infrastructures constructed on expansive clay. As a result of extreme rainfall events, highway slopes containing highly expansive Yazoo clay in Mississippi, U.S., become vulnerable to volume change. The phenomenon creates perched water zones within the slopes and poses a risk of slope failure. The soil-water characteristic curve (SWCC) defines the relationship between water content and soil suction, which can be obtained from different laboratory procedures. However, conventional laboratory methods have some limitations. To address this, various analytical and predictive models have been developed, but they can only offer estimates based on soil characteristics and lack seasonal variations occurring in field conditions. Studying seasonal SWCC through field measurements can help understand soil responses to changing moisture conditions. The current study utilized field data from six highway slopes in Mississippi and classified the data into different seasons: spring, summer, and fall. After obtaining van Genuchten parameters from the fitted curve for each season, the finite element method was applied to evaluate the parameters for accurate numerical analysis of infrastructures containing expansive clay. The study observed the variations in flow parameters with seasonal change that cannot be achieved when data from only one season is considered. The findings underscore the importance of field instrumentation data for developing SWCC and the significance of seasonal flow parameters in infrastructure design. 

Due to cyclic wetting and drying, the hydro-mechanical behavior of unsaturated soil is impacted significantly. In order to assess the soil strength parameters, knowing the unsaturated behavior is important. Soil moisture content is an important parameter that can define the shear strength of the soil. Most of the highway slopes of Mississippi are built on highly expansive clay. During summer, the evaporation of moisture in the soil leads to shrinkage and the formation of desiccation cracks, while during rainfall, the soil swells due to the infiltration of water. In addition to this, the rainwater gets trapped in these cracks and creates perched conditions, leading to the increased moisture content and reduced shear strength of slope soil. The increased precipitation due to climate change is causing failure conditions on many highway slopes of Mississippi. Vetiver, a perennial grass, can be a transformative solution to reduce the highway slope failure challenges of highly plastic clay. The grass has deep and fibrous roots, which provide additional shear strength to the soil. The root can uptake a significant amount of water from the soil, keeping the moisture balance of the slope. The objective of the current study is to assess the changes in moisture contents of a highway slope in Mississippi after the Vetiver plantation. Monitoring equipment, such as rain gauges and moisture sensors, were installed to monitor the rainfall of the area and the moisture content of the soil. The data showed that the moisture content conditions were improved with the aging of the grass. The light detection and ranging (LiDAR) analysis was performed to validate the field data obtained from different sensors, and it was found that there was no significant slope movement after the Vetiver plantation. The study proves the performance of the Vetiver grass in improving the unsaturated soil behavior and stability of highway slopes built on highly expansive clay. 

Abstract Stimuli‐responsive biomaterials show great promise for modeling disease dynamics ex vivo with spatiotemporal control over the cellular microenvironment. However, harvesting cells from such materials for downstream analysis without perturbing their state remains an outstanding challenge in 3/4‐dimensional (3D/4D) culture and tissue engineering. In this manuscript, a fully enzymatic strategy for hydrogel degradation that affords spatiotemporal control over cell release while maintaining cytocompatibility is introduced. Exploiting engineered variants of the sortase transpeptidase evolved to recognize and selectively cleave distinct peptide sequences largely absent from the mammalian proteome, many limitations implicit to state‐of‐the‐art methods to liberate cells from gels are sidestepped. It is demonstrated that evolved sortase exposure has minimal impact on the global transcriptome of primary mammalian cells and that proteolytic cleavage proceeds with high specificity; incorporation of substrate sequences within hydrogel crosslinkers permits rapid and selective cell recovery with high viability. In composite multimaterial hydrogels, it is shown that sequential degradation of hydrogel layers enables highly specific retrieval of single‐cell suspensions for phenotypic analysis. It is expected that the high bioorthogonality and substrate selectivity of the evolved sortases will lead to their broad adoption as an enzymatic material dissociation cue and that their multiplexed use will enable newfound studies in 4D cell culture.